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Surana, S. J.
- Evaluation of Anti-oxidant and Anti-inflammatory Activity of Stem Bark of Bridelia airy- shawii in Rats
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PDF Views:429
Authors
Source
Journal of Natural Remedies, Vol 8, No 1 (2008), Pagination: 18-23Abstract
Objective: To investigate antioxidant and anti-inflammatory activity of the petroleum ether, chloroform, methanol and aqueous extracts of Stem Bark of Bridelia airy - shawii (Li). Materials and Methods: The anti-inflammatory activity of the petroleum ether, chloroform, methanol and aqueous extracts of the stem bark of Bridelia airy - shawii. (Euphorbiaceae) were evaluated by using carrageenan induced rat paw edema and cotton pellet granuloma method in rat. The anti oxidant activity of all the extracts was determined by using Nitric oxide scavenging assay. Results: It was observed that, all the extracts significantly inhibited the carrageenan induced rat paw edema at the dose of 200 mg/kg b.w. orally, when compared with control and standard drug Ibuprofen. Extracts also suppressed the granulomatous tissue formation of chronic inflammation, when compared with standard drug Diclofenac sodium. The stem bark of B. airy - shawii extracts showed significant free radical scavenging activity against nitric oxide (NO.) induced release of free radicals. Conclusion: Therefore, present study suggests, potential of stem bark of B. airy shawii in inhibition of both acute and chronic phases of an inflammatory process.Keywords
Bridelia airy - shawii, Carrageenan Induced Edema, Cotton Pellet Granuloma, Nitric Oxide Inhibition- Determination of Bioequivalence of Two Oral Formulations of Gatifloxacin Tablets in Healthy Male Volunteers Using Urinary Excretion Data
Abstract Views :248 |
PDF Views:2
Authors
V. G. Kuchake
1,
P. H. Patil
2,
H. S. Mahajan
2,
P. V. Ingle
2,
S. J. Surana
3,
M. M. Thakare
3,
S. Sudharshini
4
Affiliations
1 Department of Clinical Pharmacy, R.C.Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
2 R.C.Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
3 R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
4 Department of Pharmacy Practice, Vaagdevi College of Pharmacy, Hannamkonda, A.P., IN
1 Department of Clinical Pharmacy, R.C.Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
2 R.C.Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
3 R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
4 Department of Pharmacy Practice, Vaagdevi College of Pharmacy, Hannamkonda, A.P., IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 1, No 2 (2009), Pagination: 73-78Abstract
The present study describes development of a sensitive and simple HPTLC method for estimation of gatifloxacin (GTN) in human urine sample. The drug was extracted using chloroform after adjusting the pH of urine to 7.0. Chloroform extract was spotted on silica gel 60 F254 TLC plate and was developed in a mixture of n-butanol /methanol/ammonia (5:1:2, v/v/v) as the mobile phase and scanned at 292 nm. The peak for GTN resolved at RF of 0.47. The method was validated in terms of linearity (200 to 1200 ng/μL), precision, specificity and accuracy. The limit of detection and limit of quantification for GTN in urine were found to be 25.64 and 77.70 ng, respectively. The average recovery of GTN from urine was 100.68%. The proposed method was applied to generate urinary excretion data for GTN after administration of two market GTN tablet formulations (400 mg, Formulation R and Formulation T) to twelve healthy human volunteers in a two- treatment, open, crossover design. Various pharmacokinetic parameters like peak excretion rate ((dXU/dt)max), time for peak excretion rate (tmax), AUC0-48, AUC0-∞, cumulative amount and % cumulative amount of GTN excreted, elimination half-life (t1/2), terminal elimination rate constant (kel) and overall elimination rate constant (K), were calculated for both the formulations. The average cumulative amounts of GTN excreted in urine after administration of Formulation R and Formulation T were found to be 304 ± 11mg (82.32 ± 2.75 % of dose) and 315.2 ± 13.50 mg (78.8 ± 3.37 % of dose), respectively. The urinary excretion profiles of GTN up to 48 h for both the formulations were found to be similar. Statistical comparison (90% confidence intervals of ratio) of various pharmacokinetic parameters of Formulation T with that of Formulation R revealed that Formulation T is bioequivalent with Formulation R.Bioequivalence.Keywords
Gatifloxacin, HPTLC, Urinary excretion, Bioequivalence.References
- Wayne PA, (2000) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grows Aerobically. NCCLS 5:12-14, 20.
- Easton PA, (2000) USP24, NF19, Mack Printing Company, 2056-2061.
- Shargel BL. et al. Applied Biopharmaceutics and Pharmacokinetics.Prentice-Hall International, 1999; USA 4: 247-279.
- Shah S, Rathod S I, Savale B, Patel J, (2002) Chromatogr.-B 767:83-91.
- Straughan AA, Melikan M, Meyer J, (1979) Pharm. Sci 68:1099-1102.
- Martin MB, Uihlein R, Ings L, Pharm Sci 1984;73:437-441.
- Shishoo SC, Shah I, Rathod S, Savale M, Vora, Int. J Pharm. 2001; 228:53-67.
- Adriana MD, Raul H, Marcela, Drug Dev Ind Pharm, 2000;26:821-828.
- Zhang X, Brian R, Michael B, So win ski, Kevin M, Gatifloxacin Pharmacokinetics in Healthy Men and Women. Journal of Clinical Pharmacology, 2006; 46(10), 1154-1162.
- Lockyer M, Al-Dgither S, Al-Gaai E, Yousuf A, (2005).
- Mornerl HK, Harlwigg H, Determination of Gatifloxacin in Human Serum and Urine by HPLC. 2000; 52:105-107.
- Brian R, Overholser, Michael B, Kays, Kevin M, Sowinski, Determination of gatifloxacin in human serum and urine by high-performance liquid chromatography with ultraviolet detection, 2003.
- Al-Dgither S, Naseeruddin Alvi S, Muhammad M, Hammami, Development and validation of an HPLC method for the determination of gatifloxacin stability in human plasma, 2003.
- RP-HPLC Assay Method for Simultaneous Determination of Perindopril Erbumine and Indapamide Combination in Bulk and Tablet Dosage Form
Abstract Views :223 |
PDF Views:149
Authors
Affiliations
1 Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist: Dhule (M.S.), 425 405, IN
1 Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist: Dhule (M.S.), 425 405, IN